The Center for Molecular Medicine and the College of Medicine sponsors four scientific cores for use by researchers at the University of Kentucky and elsewhere. These cores include a Protein core providing services for the production and characterization of proteins, a Microscopy core providing training and consultation for students, staff and faculty in the use of our microscopes, an Organic Synthesis core synthesizing small molecules not commercially available, and a Genetic Technologies core producing recombinant virus, and other molecular biological reagents. These cores, sponsored in part by a Center Of Biological Research Excellence grant, through the National Institute of General Medicine, provide low-cost services to facilitate the research of its users. For University of Kentucky faculty, the core services charges are further reduced through supplementation.
Genetic Technologies: Michael Mendenhall
The Advanced Genetic Technologies Core provides a range of services related to the manipulation and delivery of nucleic acids to biological systems. Services include but are not limited to: Production and titering of recombinant viral vectors, subcloning, tagging of open reading frames, development of plasmids and/or RNAs for CRISPR/Cas manipulation, piggyBac transposon delivery systems, real-time PCR analysis, recombineering, in vitro transcription
Organic Synthesis: David Watt
The Organic Synthesis Core provides biological investigators with access to “small” organic compounds that allow investigators at the University of Kentucky and other institutions to probe important biological questions relevant to the molecular basis of human medicine.
Protein: Louis Hersh
The Protein Core facilitates recombinant protein production and characterization of macromolecules, making available well-maintained equipment and instrumentation not normally found within individual laboratories. The mission of the Protein Core is to provide training and access to its facilities for members of the Center as well as the research community in general.
Microscopy: Carole Moncman
With the growing use of recombinant fluorescent proteins for cellular biochemical analyses, microscopy has become an essential tool for biochemists. The microscopy core houses four epifluorescent microscopes and an A1R confocal microscope. All of the microscopes are equipped with digital cameras for image acquisition. The goal of this core is to provide training and consultation for students, staff, and faculty in the use of our microscopes.