The Center for Molecular Medicine sponsors scientific cores for use by researchers at the University of Kentucky and elsewhere. These include a Protein core providing services for the production and characterization of proteins as well as the production of single chain antibodies (nanobodies), a Microscopy core providing training and consultation for students, staff and faculty in the use of the available microscopes, an Organic Synthesis core synthesizing small molecules not commercially available, and a Genetic Technologies core producing recombinant virus, and other molecular biological reagents.
Genetic Technologies: Michael Mendenhall
The Advanced Genetic Technologies Core provides a range of services related to the manipulation and delivery of nucleic acids to biological systems. Services include but are not limited to: Production and titering of recombinant viral vectors, subcloning, tagging of open reading frames, development of plasmids and/or RNAs for CRISPR/Cas manipulation, piggyBac transposon delivery systems, real-time PCR analysis, recombineering, in vitro transcription
Organic Synthesis: David Watt
The Organic Synthesis Core provides biological investigators with access to “small” organic compounds that allow investigators at the University of Kentucky and other institutions to probe important biological questions relevant to the molecular basis of human medicine.
Protein: Louis Hersh
The Protein Core facilitates recombinant protein production and characterization of macromolecules, making available well-maintained equipment and instrumentation not normally found within individual laboratories. In addition the core produces single domain antibodies for research purposes. The mission of the Protein Core is to provide training and access to its facilities for members of the Center as well as the research community in general.
Microscopy: Carole Moncman
With the growing use of recombinant fluorescent proteins for cellular biochemical analyses, microscopy has become an essential tool for biomedical research. The microscopy core houses four epifluorescent microscopes and an A1R confocal microscope. All of the microscopes are equipped with digital cameras for image acquisition. The goal of this core is to provide training and consultation for students, staff, and faculty in the use of our microscopes.