Genetic Technologies Core

About the Core:

The Advanced Genetic Technologies Core provides a range of services related to the manipulation and delivery of nucleic acids to biological systems. Services include, but are not limited to:

  • Production and titering of recombinant viral vectors, including
    • Lentiviruses
    • Adenoviruses
    • Adeno-associated viruses
  • Subcloning
  • Tagging of open reading frames
  • Development of plasmids and/or RNAs for CRISPR/Cas manipulation
  • piggyBac transposon delivery systems
  • Real-time PCR analysis
  • Recombineering
  • In vitro transcription  

 The Core maintains an extensive stock of plasmids that provide components for building constructs envisioned by the investigator and provides consultative services to devise an efficient plan for their realization. We can also provide monoclonal antibodies directed against the HA, myc, and FLAG tags.

The Core also provides training opportunities in the technologies described above, either as a for-credit, one semester class or on a project-related, fee-based basis.

Current charges for the services provided by the Core are summarized below. Projects requiring the purchase of specialized reagents not currently available in the Core may incur additional material fees. UK faculty and staff are supplemented to the extent of 75% by the COBRE P30 grant. Users outside UK will pay the listed fee. 

To apply to use the Genetic Technologies Core, or for further information, contact: Michael Mendenhall.

Resources:

SnapGene®

The Genetic Technologies and Protein cores are now offering a service called SnapGene. SnapGene is a program which allows researchers to to plan, visualize, and document their molecular biology procedures. To learn more about the program, go to: 

https://www.snapgene.com

Important: This service is only available to University of Kentucky researchers.


For access to SnapGene, please contact Dr. Michael Mendenhall.

Services:

**Projects that require special materials will incur an additional cost for the purchase of such materials