Protein Core Services

Protein Services

Protein Expression and Affinity Purification from E. coli ( 1liter)

Internal Rate

 $1,150

 External Rate

 $1,750

Protein Expression and Affinity Purification in Insect Cells (baculovirus 200 ml)

 Internal Rate

 $2,400

 External Rate

 $3,600

Protein Expression and Affinity Purification in Mammalian Cells (100 ml)

 Internal Rate

 $2,500

  External Rate

 $3,250

Each additional Purification Step (E. coli. baculovirus, or mammalian cells)

Internal Rate

$500

External Rate 

$750 

Gene synthesis including mutagenesis (codon optimized)

 Interanl Rate

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External Rate 

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Characterization of Macromolecule by Dynamic Light Scattering

 Internal Rate

 $250

External Rate 

$375

Circular Dichroism Spectrum

 Internal Rate

 $250

 External Rate

 $375

Thermal Melt Curve

 Internal Rate

 $300

Non-Academic Rate 

$450 

Characterization of Binding by Isothermal Titration-Calorimetry

 Internal Rate

 $550

  External Rate 

$788 

Ligand Screening by Differential Scanning Fluorometry

 Internal Rate

 $400

  External Rate 

 $600

SDS-PAGE

 Internal Rate

 $175

  External Rate 

$265

Western Blot with User Supplied or Common Antibody

 Internal Rate

 $200

  External Rate 

$300

Nanobody Production (1 mg purified nanobody, sequence, and clone)

Internal Rate 

 $6,000

External Rate 

$9,000

Additional Purified Nanobody (per mg)

Internal Rate 

 $400

External Rate 

$600

Additional Validated Nanobody Clones

Internal Rate 

 $400

External Rate 

 $600

Phagemid Librray From Which Nanobody was Isolated

 Internal Rate

 $750

External Rate 

$975 

Expression Clone Fused to Reporter

 Internal Rate

 $400

   External Rate 

$600

 Internal Rate

 $2,500

   External Rate 

$3,500

Nanobody Clone Isolated From Naïve Alpaca Library

 Internal Rate

 $2,000

  External Rate 

$3,000

Maturation of Nanobody From Naïve Alpaca Library.

 Internal Rate

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  External Rate 

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Instrument Use Charges

Hourly Rates

Special Projects

 Internal Rate

 $82.50/hr

External Rate 

$125/hr 

Training and Consulting

 Academic Rate

 $82.50/hr

 Non-Academic Rate

$125/hr 

Instruments Available in the Protein Core

Cell Culture

New Brunswick BioFlow 3000 10 liter fermenters

Tissue culture facility for mammalian and insect cells

Wheaton roller bottle apparatus for adherent culture

Wheaton Microstir 4PL and spinner flasks for suspension culture

Protein Purification

Microfluidics 110Y high volume cell cracking device

GE Healthcare AKTA chromatography systems with associated columns and resins

Analytical Instrumentation

Waters Breeze high performance liquid chromatography apparatus

Jasco J-810 spectropolarimeter

Perkin-Elmer LS55 spectrofluorometer

Molecular Devices Spectra Max fluorescent plate readers

MicroCal VP-ITC isothermal titration calorimeter

Wyatt NanoStar dynamic light scattering instrument

Structural Biology

TTP Labtech Mosquito Crystallization Screening Robot

Rigaku Minstrel DT UV automated imager

SER-CAT macromolecular data collection facility at the Advanced Photon Source, Argonne National Lab

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