Nanobody Production

NanobodyOrderForms 2020.pdfNanobodyOrderForm S2020.pdfNanobodyOrderForm AW 2.pdf

Nanobodies®: The "hot" new research tool.

We will make Nanobodies® for you.

About Nanobody Production

Single domain antibodies (nanobodies) are increasingly becoming popular as reagents for biomedical research, for use as therapeutics, and as reagents for diagnostics. The number of publications citing antibodies is increasing each year with over 2,000 publications cited in PubMed to date. This is due, in large part, to their small size, stability, high affinity, high specificity, ease of manipulation, and ease of production.  

Through resources provided by the National Institute of General Medical Sciences, our University based Protein Core is producing single domain antibodies in alpacas on a fee for service structure. Our facility immunizes alpacas with your protein of interest, amplifies the produced pool of nanobody cDNAs by PCR and generates a phagemid library, which is then screened by panning against your protein. Positive clones are sequenced, with nanobodies to your target confirmed by ELISA. The nanobodies to your protein are produced and purified from E. coli with 1 mg of purified nanobody, the nanobody clone, as its sequence as deliverables. To date we have successfully generated more than 75 nanobodies. These nanobodies are for research purposes.

In many cases our core can produce your protein for nanobody production by expressing it in E. coli, insect cells, or mammalian cells and purifying it.

We are now taking orders for producing nanobodies starting in November, 2020. The cost to produce a nanobody is $9,000. To produce a nanobody to your protein we need ~1.5 mg of purified protein in PBS or HBS at a concentration of 1 mg/ml or higher. We typically deliver your nanobody within ~3 – 4 months. Usually we identify more than one nanobody against a particular protein which can be purchased for a nominal cost. A range of derivatives and conjugates are also available. 

To request a nanobody produced to your protein please fill out and return this request form:

Nanobody Production Request Form

or contact Lou Hersh at lhersh@uky.edu or 859-536-0034.

For the forthcoming nanobody production cycle we need to receive your purified protein by October 23rd, 2020.

 

 

EXAMPLES OF NANOBODY USES

SUGGESTED READING

Immunization of Alpacas (Lama Pacos) with Protein Antigens and Production of Antigen-specific Single-Domain Antibodies. K. Martin Chow, Sidney W. Whiteheart, Jeffrey R. Smiley, Savita Sharma, Kathy Boaz, Meggie J. Coleman, Alvina Maynard, Louis B. Hersh, Craig W. Vander Kooi. JOVE. 2019 (in press) 

Nanobody approval gives domain antibodies a boost.  Morrison C. Nat Rev Drug Discov. 2019 Jul;18 (7) : 485-487

Exploiting Nanobodies' Singular Traits. Ingram JR, Schmidt FI, Ploegh HL. Annu Rev Immunol. 2018 Feb 28.

Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2126-2131.

Editorial: Single-Domain Antibodies-Biology, Engineering and Emerging Applications. Henry KA, MacKenzie CR. Front Immunol. 2018 Jan 23;9:41.

Nanobodies as novel agents for cancer therapy. Revets H, De Baetselier P, Muyldermans S. Expert Opin Biol Ther. 2005 Jan;5(1):111-24. Review

Nanobodies and their potential applications. Hassanzadeh-Ghassabeh G, Devoogdt N, De Pauw P, Vincke C, Muyldermans S.  Nanomedicine (Lond). 2013 Jun;8(6):1013-26.

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