Protein Core Services

Protein Services

Protein Expression and Affinity Purification from E. Coli (1liter)

Internal Rate

  $1,150

 External Rate

$1,750

Protein Expression and Affinity Purification in Insect Cells (Baculovirus 200 ml)

 Internal Rate

   $2,400

 External Rate

 $3,600

Protein Expression and Affinity Purification in Mammalian Cells (100 ml)

 Internal Rate

   $2,500

  External Rate

 $3,250

Each additional Purification Step (E. Coli. Baculovirus, or Mammalian Cells) 

Internal Rate

  $500

External Rate 

$750 

Gene Synthesis Including Mutagenesis (Codon Optimized)

 Internal Rate

  Quote

   External Rate 

Quote

Characterization of Macromolecule by Dynamic Light Scattering

 Internal Rate

  $250

   External Rate 

$375

Circular Dichroism Spectrum

 Internal Rate

   $250

 External Rate

 $375

Thermal Melt Curve

 Internal Rate

 $300

   External Rate 

$450 

Characterization of Binding by Isothermal Titration-Calorimetry

 Internal Rate

  $550

  External Rate 

$825

Ligand Screening by Differential Scanning Fluorometry

 Internal Rate

 $400

  External Rate 

 $600

SDS-PAGE

 Internal Rate

  $175

  External Rate 

$265

Western Blot with User Supplied or Common Antibody

 Internal Rate

  $200

  External Rate 

$300

Nanobody Production (1 mg Purified Nanobody, Sequence, and Clone)

Internal Rate 

  $6,000

External Rate 

$9,000

Additional Purified Nanobody (Per mg)

Internal Rate 

  $400

External Rate 

$600

Additional Validated Nanobody Clones*

Internal Rate 

   $400

External Rate 

 $600

*  *From clones previously identified as positive clones

Additional Validated Nanobody Clones*

Internal Rate 

   $600

External Rate 

 $900

* From clones previously sequenced but not validated

Phagemid Librray From Which Nanobody was Isolated

Internal Rate

 $750

 External Rate 

$975 

Expression Clone Fused to Reporter

 Internal Rate

  $400

   External Rate 

$600

Nanobody Library in Phage Display Vector From Alpacas Immunized With Supplied Antigen

 Internal Rate

  $2,500

   External Rate 

$3,500

Nanobody Clone Isolated From Naïve Alpaca Library

 Internal Rate

  $4,000

  External Rate 

$6,000

Maturation of Nanobody From Naïve Alpaca Library

 Internal Rate

  quote

  External Rate 

quote

Hourly Rates

Special Projects

 Internal Rate

    $82.50/hr

  External Rate 

$125/hr 

Training and Consulting

 Academic Rate

  $82.50/hr

 Non-Academic Rate

$125/hr 

Instruments Available in the Protein Core

Cell Culture

  • New Brunswick BioFlow 3000 10 Liter Fermenters
  • Tissue Culture Facility for Mammalian and Insect Cells
  • Wheaton Roller Bottle Apparatus for Adherent Culture
  • Wheaton Microstir 4PL and Spinner Flasks for Suspension Culture

Protein Purification

  • Microfluidics 110Y High-Volume Cell Cracking Device
  • GE Healthcare AKTA Chromatography Systems With Associated Columns and Resins

Analytical Instrumentation

  • Waters Breeze High-Performance Liquid Chromatography Apparatus
  • Jasco J-810 Spectropolarimeter
  • Perkin-Elmer LS55 Spectrofluorometer
  • Molecular Devices Spectra Max Fluorescent Plate Readers
  • MicroCal VP-ITC Isothermal Titration Calorimeter
  • Wyatt NanoStar Dynamic Light Scattering Instrument

Structural Biology

  • TTP Labtech Mosquito Crystallization Screening Robot
  • Rigaku Minstrel DT UV Automated Imager
  • SER-CAT Macromolecular Data Collection Facility at the Advanced Photon Source, Argonne National Lab

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