Protein Core Services

Protein Services

Pilot Bacterial Expression

 Academic Rate

 $450

 Non-Academic Rate

 $675

Initial Setup and Test Expression in Yeast

 Academic Rate

 $1,200

 Non-Academic Rate

 $1,800

Initial Setup and Test Expression in Insect Cells

 Academic Rate

 $1,800

 Non-Academic Rate

 $2,700

Initial Setup and Test Expression in Mammalian Cells

 Academic Rate

 $1,000

 Non-Academic Rate

 $1,500

Bacterial Culture (First Liter)

 Academic Rate

 $400

 Non-Academic Rate

 $600

Bacterial Culture (Additional Liter)

 Academic Rate

 $75

 Non-Academic Rate

 $113

Yeast Culture (First Liter)

 Academic Rate

$500 

Non-Academic Rate 

$750 

Yeast Culture (Additional Liter)

 Academic Rate

$225 

Non-Academic Rate 

$338 

Insect Cell Culture (Standard 200ml)

 Academic Rate

 $400

Non-Academic Rate 

 $600

Mammalian Cell Culture

Quote from professor required.

Purification – Ion Exchange 1ml Column

 Academic Rate

$340 

Non-Academic Rate 

$510 

Purification – Ion Exchange 5ml Column

 Academic Rate

$450 

Non-Academic Rate 

$675 

Purification – Gel Filtration

 Academic Rate

 $280

Non-Academic Rate 

$420 

Purification Affinity

 Academic Rate

$500 

Non-Academic Rate 

$750 

Purification Hydrophobic Chromatography <5ml

 Academic Rate

$450 

Non-Academic Rate 

$675 

PCR Cloning

 Academic Rate

 $1,125

Non-Academic Rate 

$1,688 

Subcloning

 Academic Rate

 $340

Non-Academic Rate 

$510 

Mutagenesis (Quick Change)

 Academic Rate

 $730

Non-Academic Rate 

$1,096 

Characterization of Macro-Molecule by Dynamic Light Scattering

 Academic Rate

 $225

Non-Academic Rate 

$338 

Circular Dichroism Spectrum

 Academic Rate

 $225

 Non-Academic Rate

 $338

Thermal Melt Curve Following Circular Dichroism

 Academic Rate

 $300

Non-Academic Rate 

$450 

Characterization of Binding by Isothermal Titration-Calorimetry

 Academic Rate

 $525

Non-Academic Rate 

$788 

Ligand Screening by Differential Scanning Fluorometry

 Academic Rate

 $375

Non-Academic Rate 

 $563

Ligand Screening Additional Plate

 Academic Rate

 $225

Non-Academic Rate 

$338 

SDS-PAGE

 Academic Rate

 $160

Non-Academic Rate 

$240 

Western Blot with User Supplied or Common Antibody

 Academic Rate

 $225

Non-Academic Rate 

$338 

Crystallization Screening (4x96 Conditions)

Academic Rate 

 $375

Non-Academic Rate 

$563 

Data Set Collection

Academic Rate 

 $450

Non-Academic Rate 

$675 

Structure Determination by Molecular Replacement

Academic Rate 

 $375

Non-Academic Rate 

 $563

Structure Determination Variant of Previously Determined Structure

 Academic Rate

 $150

Non-Academic Rate 

$225 

Structure Refinement and Analysis

Quote from professor required.

Instrument Use Charges

Crystallization Robot Use with Core Seals, Strips, Tips

 Academic Rate

 $15/use

Non-Academic Rate 

$23/use 

Crystallization Robot Use with Core Seals Strips, Tips, Plates

 Academic Rate

 $17/use

 Non-Academic Rate

$26/use 

Use of Core Crystallization Screens (Per 96 Solution Screen)

 Academic Rate

 $10/use

 Non-Academic Rate

$15/use 

Hourly Rates

Special Projects

 Academic Rate

 $75/hr

Non-Academic Rate 

$113/hr 

Training and Consulting

 Academic Rate

 $75/hr

 Non-Academic Rate

$113/hr 

Instruments Available in the Protein Core

Cell Culture

New Brunswick BioFlow 3000 10 liter fermenters

Tissue culture facility for mammalian and insect cells

Wheaton roller bottle apparatus for adherent culture

Wheaton Microstir 4PL and spinner flasks for suspension culture

Protein Purification

Microfluidics 110Y high volume cell cracking device

GE Healthcare AKTA chromatography systems with associated columns and resins

Analytical Instrumentation

Waters Breeze high performance liquid chromatography apparatus

Jasco J-810 spectropolarimeter

Perkin-Elmer LS55 spectrofluorometer

Molecular Devices Spectra Max fluorescent plate readers

MicroCal VP-ITC isothermal titration calorimeter

Wyatt NanoStar dynamic light scattering instrument

Structural Biology

TTP Labtech Mosquito Crystallization Screening Robot

Rigaku Minstrel DT UV automated imager

SER-CAT macromolecular data collection facility at the Advanced Photon Source, Argonne National Lab

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