The Protein Core facilitates recombinant protein production and characterization of macromolecules, making available well-maintained equipment and instrumentation not normally found within individual laboratories. In addition, this core provides a single chain antibody (nanobody) production service. The mission of the Protein Core is to provide training and access to its facilities for members of the Center as well as the research community in general. Projects are undertaken on a service basis. A full time staff member maintains the core equipment, provides training to new users, works with researchers to make the best use of the instrumentation, and oversees scheduling.
The Core has the capabilities to assist researchers in moving their project from protein expression to biophysical characterization to protein crystallization. The core assists in cell culture, protein production and purification, and macromolecular characterization through a number of techniques. Nanobodies are produced in alpacas through the core. Researchers can make use of core instrumentation on a regular basis unassisted by core personnel after initial training. Core personnel offer training on all instruments and equipment, as well as a higher-level of assistance when projects warrant. Additionally, service projects are undertaken, including protein production, using heterologous expression in bacteria, insect, or mammalian cells, and protein purification.
Current charges for the services provided by the Cores are listed below.
To apply to use the Protein Core, or for further information, click here:Apply to Core
The Genetic Technologies and Protein Cores are now offering a service called SnapGene. SnapGene is a program which allows researchers to plan, visualize, and document their molecular biology procedures. To learn more about the program, go to:
Important: This service is only available to University of Kentucky researchers.
For access to SnapGene, please contact Dr. Michael Mendenhall.